In physics, velocity refers to the rate at which something changes in position over time.
In biochemistry, velocity can be used to refer to the amount of product formed over time and is often used synonymously with rate of reaction. The temperature and pressure in the room won't change.
With these factors constant, the only factor left affecting the rate is the amount of substance used or product formed.
Thus, the rate of a reaction can be calculated by multiplying the rate constant by the substrate concentration (amount) or by determining reaction velocity (V).
In this example, the rate of reaction would be how fast hydrogen and oxygen combine to form water.
Of course, the reverse reaction also has a rate as well: how fast water is changed into hydrogen and oxygen.
Enzymes bind substrate at the active site and change them into products.The concentration of your substrate then is 10 Molar (10M).Eventually, no matter how many additional ingredients you throw in, the electric mixer won't make cake batter any faster than it already is, unless you add yet another beater.How much the enzyme likes the substrate is known as the affinity, or Km, and it is calculated by looking at the rate constants of all reactions involved.The Michaelis-Mention Equation is where the initial velocity equals the Vmax multiplied by the substrate concentration and divided by sum of Km and the substrate concentration. A reaction rate is the speed at which one substance changes into another.The reaction rate, also known as reaction velocity, is the measure of the formation of product over time.